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ELISA Pig-MAP

Detect problems of health and poor welfare in your animals with high sensitivity testing Pig-MAP. Acuvet ELISA pig-MAP is a pig specific, sensitive and reliable test to determine this biomarker using basic equipment (ELISA reader).

 

Do you want to determine the level of stress in pig? Do you want to have a quantitative indicator of animal welfare that is objective and easy to determine?
Do you want to know the degree of inflammation caused by vaccines or weaning?
Do you want to measure Pig-MAP with a sensitive and reliable method?

 

HOW DOES IT HELP YOU?

 

Pig-MAP is a sensitive and quantitative marker to assess the on-farm animal health and welfare. It can also be used to quantify inflammatory processes in your experimental trials, for example evaluation of vaccines, or nutraceutical products. The Acuvet ELISA pig-MAP is a reliable method that uses specific antibodies and standards for swine. With high sensitivity to quantify this biomarker not only in serum but in other matrices such as meat juice. It is calibrated according to the European Reference Serum for Porcine Acute Phase Proteins. This is very important when comparing your results with the main reference studies available in the scientific literature.

MATRIX
PLASMA

MEAT JUICE

APPLICATIONS AND USERS
LABORATORY

ASSAY PROCEDURE

1. Sample:

Incubation RT

2. Conjugate:

Wash and apply conjugated.

Incubation at room temperature.

Wash and add substrate.

3. Result:

Photometrical reading at 45 nm

Nombre del video

1. Sample:

Dilute sample/standard and apply

Incubate RT

2. Conjugate:

Incubation

3. Results:

Photometrical reading at 45 nm

TEST PRINCIPLE

Acuvet ELISA pig-MAP is a sandwich-type ELISA, based on two monoclonal antibodies specific for Pig-MAP. The assay is calibrated according to the European Reference Serum for pig Acute Phase Proteins (EU concerted Action QLK5-CT-1999-0153)

TECHNICAL DATA

  • Specific for swine
  • Limit of detection: 0.2 μg/mL
  • Working range: 0.3-3 μg/mL
  • Time of assay: 90 minutes

 

Validations:

Internal validation following the AOAC and Eurachem guidelines.

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SKU: ELISA PIG-MAP-ESP Categories: ,